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IgG1 (A) and IgG2a (B) isotype responses in mice vaccinated with Ag2/PRA(1-194) (○), Ag2/PRA(19-194) (▪), Ag2/PRA(1-18) DNA (▵), or the pVR1012 vector alone (•), as measured by an ELISA using full-length GST-Ag2/PRA recombinant protein as the target antigen. Results are mean antibody levels in assays of sera pooled from groups of 10 mice 12 days after i.p. challenge with 2,500 arthroconidia. Similar results were obtained in three independent experiments.
