FIG. 4.

Contribution of T cells to production of IFN-γ and IL-10. Mice were immunized with rHsp60 and depleted of CD4⁺ or CD8⁺ cells or both subsets, and at serial intervals following injection, splenocytes were exposed to rHsp60 or to medium. The levels of cytokines in supernatants from cells were determined by ELISA. The results represent means ± SEM for six mice at each time point. The arrows on the x axes indicate the time of the second injection of antigen. #, P < 0.01 compared to controls.