FIG. 10.

Predicted stem-loop structure of RNA derived from the pilT-pilU intergenic sequences generated by the FOLD program of the Wisconsin Genetics Computer Group package. The RNAs corresponding to the sense strand of the intergenic regions have optimal secondary structures with minimal free energies of −53.2 kcal/mol (length, 162 bp) for N. gonorrhoeae (A) and −64.1 kcal/mol (length, 171 bp) for P. aeruginosa (B). The numbering of nucleotides is as in GenBank accession number S72391 for panel A and M55524 for panel B. The asterisks indicate the stop codon of the pilT ORFs, and the dots indicate putative ribosome binding sites for pilU.