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Quantitative analysis of Tfp and PilC in pilU mutants. The amounts of samples loaded were equalized based on the total protein concentration of whole cells from which Tfp were purified. (Top) Immunoblot of purified pili obtained by using rabbit antibodies specific for PilC. (Bottom) Coomassie blue-stained SDS-PAGE gel showing the relative amounts of PilE in purified pili.
