TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| N. gonorrhoeae strains | ||
| N400 | Derived from VD300,a contains the IPTG-inducible recA6 alleleb | 11 |
| GF2 (PilF−) | pilF::m-Tn3erm | 11 |
| GT3 | pilT::m-Tn3erm | 40 |
| GT7 | pilT::m-Tn3erm | 40 |
| GT17 | pilT::m-Tn3cm at position 73c | This study |
| GT26 | pilT::m-Tn3erm | 40 |
| GT50 | pilT::m-Tn3erm at position 1105c | This study |
| GT101 | pilTdud1 | 40 |
| GT102 | pilTΔQSL | 40 |
| GT103 | pilT::m-Tn3erm | 40 |
| GT104 | pilTind | 40 |
| GT105 | pilTfs164 | 40 |
| GTU2 | pilT::m-Tn3cm pilU::mTn3erm | This study |
| GTXE1 | pilT::xylE | This study |
| GUXE2 | pilU::xylE | This study |
| GU2 | pilU::m-Tn3erm at position 1591c | This study |
| GU4 | pilU::m-Tn3erm at position 1714c | This study |
| GU5 | pilU::m-Tn3erm at position 2102c | This study |
| GU21 | pilU::m-Tn3erm at position 1314c | This study |
| GU33 | pilU::m-Tn3erm at position 2541c | This study |
| GU127 | pilU::m-Tn3erm at position 1192c | This study |
| E. coli strains | ||
| BL21(DE3) | F−ompT rB− mB− with prophage λ carring the T7 RNA polymerase gene | 32 |
| HB101 | F−mcrB mrr hsdS20(rB− mB−) recA13 supE44 ara14 galK2 lacY1 proA2 rpsL20(Smr) xyl5 λ−leu mtl1 | GIBCO BRL |
| Plasmids | ||
| pBluescript II SK | Cloning vector, Ampr | 32 |
| p6/16/11 | 1.1-kb SalI fragment in pBluescript II SK | This study |
| p11/2/13 | 3.3-kb EagI/XbaI fragment in pBluescript II SK | This study |
| pHSS6 | Vector for shuttle mutagenesis, Kanr | 27 |
| p1-49 | 3.3-kb EagI/XbaI fragment in pHSS6 | This study |
| pT7-5 | T7 RNA polymerase promoter λ10, Ampr | 32 |
| pT7U | pilU in pT7-5 | This work |
| pT7U(ΔSalI) | SalI deletion subclone of pT7U | This work |