FIG. 1.

Cytofluorimetric analysis of C5aR expression on PMN. PMN were incubated for 30 min in the absence (black histograms) or in the presence (grey lines) of (i) heat-killed acapsular cryptococci (7698) (E/T ratio, 1:4) (B), (ii) live 7698 (E/T ratio, 1:4) (C), (iii) heat-killed encapsulated cryptococci (6995) (E/T ratio, 1:4) (D), or (iv) live 6995 (E/T ratio, 1:4) (E). (A) Control showing autofluorescence of the cells (dotted line) and staining profile with an irrelevant antibody (black line). The results are from one experiment representative of four with similar results, performed by using cells from four different healthy subjects. FL1-H, fluorescent channel 1 height.