FIG. 2.

Cytofluorimetric analysis of C5aR expression on PMN with GXM. PMN were incubated for 30 min in the absence (black histograms) or in the presence (grey lines) of (i) heat-killed acapsular cryptococci (7698) (E/T ratio, 1:4) (B), (ii) GXM alone (250 μg/ml) (C), or (iii) 7698 cells (E/T ratio, 1:4) pretreated with GXM (D). (A) Control showing autofluorescence of the cells (dotted line) and staining profile with an irrelevant antibody (black line). The results are from one experiment representative of four with similar results, performed by using cells from four different healthy subjects. (E) Mean fluorescence intensity for all groups. The results are the mean and standard error of the mean for four independent experiments with cells from four different healthy subjects. Statistical significance was determined by using ANOVA with Bonferroni's posttest analysis. FL1-H, fluorescent channel 1 height.