FIG. 1.

Expression of TLR, MyD88, and MD-2 in human hepatocytes by RT-PCR. (Top panel) Total RNA from both cultured human primary hepatocytes and HepG2 cells were reverse transcribed with an oligo(dT) primer, followed by PCR with primers specific to TLR1 to TLR9, MyD88, or β-actin (as a loading control). (Bottom panel) Total RNA from freshly isolated hepatocytes from saline- or LPS-injected (2.5 mg/kg of body weight) (intraperitoneally [i.p.] administered at 24 h) C3H/HeN or C3H/HeJ mice were subjected to RT-PCR and analysis of MD-2 and β-actin.