FIG. 4.

NF-κB activation by LPS in mouse hepatocytes infected with Ad.mTLR4. (Top panel) Hepatocytes from C3H/HeN or C3H/HeJ mice were infected with Ad.mTLR4 (multiplicity of infection = 10) or Ad.LacZ for 3 h and recovered overnight. At 24 h after the start of infection, cells were treated with the indicated concentrations of LPS for 60 min. Nuclear extracts were prepared, and the binding of nuclear proteins to NF-κB consensus oligonucleotide was analyzed by EMSA. Data are representative of three independent experiments. (Bottom panel) Total RNA (10 μg per sample) was subjected to Northern blot analysis and hybridized with a ³²P-labeled rat TLR4 cDNA.