TABLE 1.

TLR RT-PCR primer sequences

Gene name	Size (nt)	Primer direction	RT-PCR sequence
Human TLR1	439	Sense	5′-AGTTGTCAGCGATGTGTTCGG-3′
		Antisense	5′-GATCAAGTACCTTGATCCTGGG-3′
Human TLR2	294	Sense	5′-GGCTTCTCTGTCTTGTGACC-3′
		Antisense	5′-GGGCTTGAACCAGGAAGACG-3′
Human TLR3	270	Sense	5′-AGCCACCTGAAGTTGACTCAGG-3′
		Antisense	5′-CAGTCAAATTCGTGCAGAAGGC-3′
Human TLR4	438	Sense	5′-TTGTATTCAAGGTCTGGCTGG-3′
		Antisense	5′-GCAACCTTTGAAACTCAAGCC-3′
Human TLR5	338	Sense	5′-AGCCATCTGACTGCATTAAGG-3′
		Antisense	5′-GACTTCCTCTTCATCACAACC-3′
Human TLR6	592	Sense	5′-CCTGGGAGGTAAACATCTGA-3′
		Antisense	5′-CCCTCAACCACATAGAAACGA-3′
Human TLR7	321	Sense	5′-GATAACAATGTCACAGCCGTCC-3′
		Antisense	5′-GTTCCTGGAGTTTGTTGATGTTC-3′
Human TLR8	309	Sense	5′-GTGTCACCCAAACTGCCAAGCTCC-3′
		Antisense	5′-GATCCAGCACCTTCAGATGAGGC-3′
Human TLR9	233	Sense	5′-TACCAACATCCTGATGCTAGACTC-3′
		Antisense	5′-TAGGACAACAGCAGATACTCCAGG-3′
Human MyD88	358	Sense	5′-CCGCGCTGGCGGAGGAGATGGAC-3′
		Antisense	5′-GCAGATGAAGGCATCGAAACGCTC-3′
Mouse TLR2	329	Sense	5′-GGCATTAAGTCTCCGGAATTATC-3′
		Antisense	5′-CCATTGAGGGTACAGTCGTCG-3′
Mouse TLR4	439	Sense	5′-GGAAGGACTATGTGATGTGACC-3′
		Antisense	5′-GCTCTTCTAGACCCATGAAATTGG-3′
Mouse MD-2	293	Sense	5′-CTGAATCTGAGAAGCAACAGTGG-3′
		Antisense	5′-CAGTCTCTCCTTTCAGAGCTCTGC-3′