TABLE 2.
DNA microarray analysis of gene expression changes in mouse hepatocytes stimulated with LPS: increased gene expressiona
| Function | Fold change | Gene product(s) and description |
|---|---|---|
| Cell death or apoptosis | 2.9 | ESTs, moderately similar to PI-3 catalytic subunit, beta isoform (Homo sapiens) |
| 1.5 | Calpastatin | |
| Liver metabolizing enzyme | 1.6 | Aldehyde dehydrogenase family 1, subfamily A4 |
| 1.6 | Selenophosphate synthetase 2 | |
| 1.6 | Cytochrome P450, 2a5 | |
| 1.6 | Alcohol dehydrogenase 5 | |
| 1.6 | Spermine synthase | |
| 1.6 | Triosephosphate isomerase | |
| 1.6 | Uridine phosphorylase | |
| 1.5 | Phosphoglycerate kinase 1 | |
| 1.5 | Mitochondrial acyl coenzyme A thioesterase | |
| 1.5 | Cytochrome P450, 2c29 | |
| 1.5 | Cytochrome P450, 2a4 | |
| 1.5 | Cytochrome P450, 2c37 | |
| 1.5 | Paraoxonase 1 | |
| Signal transduction | 2.4 | Inhibitor of DNA binding 2 |
| 1.7 | RFG | |
| 1.6 | Mus musculus calcium/calmodulin-dependent protein kinase II delta mRNA, partial cds | |
| 1.5 | Protein tyrosine phosphatase, nonreceptor type 13 interacting protein | |
| 1.5 | Signal recognition particle, 9 kDa | |
| 1.5 | ESTs, highly similar to putative adenosine kinase (Saccharomyces cerevisiae) | |
| 1.5 | Protein kinase, interferon-inducible double-stranded RNA-dependent inhibitor | |
| 1.5 | Disabled homolog 1 (Drosophila) | |
| 1.5 | T-cell-specific GTPase | |
| 1.5 | Aplysia ras-related homolog N (RhoN) | |
| 1.5 | Bright and dead ringer gene product homologous protein Bdp | |
| Stress response or antioxidant | 1.9 | Glutathione-S-transferase, alpha 3 |
| 1.8 | Glutathione-S-transferase, alpha 2 (Yc2) | |
| 1.6 | Glutathione-S-transferase, alpha 4 | |
| 1.6 | Glutathione-S-transferase, mu 6 | |
| 1.5 | Glutathione transferase, zeta 1 (maleylacetoacetate isomerase) | |
| 1.6 | Selenoprotein P, plasma, 1 | |
| Liver protein or acute-phase protein | 1.7 | Serum albumin variant |
| 1.6 | Selenium binding protein 1 | |
| 1.6 | Group-specific component | |
| 1.6 | Complement component 4 binding protein | |
| 1.6 | ESTs, highly similar to fibrinogen gamma-a and -b chain precursors (Rattus norvegicus) | |
| 1.6 | ESTs, highly similar to insulin-induced growth response protein cl-6 (Rattus norvegicus) | |
| 1.5 | Complement component factor I | |
| 1.5 | Apolipoprotein H | |
| 1.5 | Sterol carrier protein 2, liver | |
| 1.5 | Orosomucoid 1 (AGP) | |
| DNA binding protein | 1.5 | Histone H2A.Z |
| Glucose response | 1.5 | Pleiotropic regulator 1, PRL1 homolog (Arabidopsis) |
| Transcription factor | 1.5 | ESTs, highly similar to hypothetical 18.5-kDa protein c12g12.05c (Schizosaccharomyces pombe) |
| 1.5 | LIM only 2 | |
| Reverse transcriptase | 1.5 | ESTs, weakly similar to open reading frame (M. musculus) |
| Cell adhesion | 1.6 | Desmoglein 2 |
| 1.5 | P40-8, functional | |
| Cysteine proteinase | 1.6 | Cathepsin L |
| Membrane protein | 1.5 | Flotillin 1 |
| 1.5 | Lymphocyte antigen 6 complex | |
| Cell locomotion | 1.5 | Hyaluronan-mediated motility receptor (RHAMM) |
| Tight junction protein | 1.5 | Claudin 1 |
| Protease inhibitor | 1.5 | Calpastatin |
a
Total RNA from cultured hepatocytes from C3H/HeN mice was subjected to DNA microarray analysis. The mRNA from normal and LPS (100-ng/ml)-treated (8 h) mice was labeled with cyanine-3-dUTP (green) and cyanine-5-dUTP (red), respectively. Positive fold change means gene expression was increased following treatment with LPS. The genes are sorted by function according to PubMed search. Only genes whose expression has been increased by at least 50% (1.5-fold) are shown here.