Skip to main content
PMC home page
Biophysical Journal logoLink to Biophysical Journal
. 1990 Mar;57(3):445–459. doi: 10.1016/S0006-3495(90)82561-3

Dynamics of phosphatidylcholine-cholesterol mixed model membranes in the liquid crystalline state.

Y K Shin 1, J K Moscicki 1, J H Freed 1
PMCID: PMC1280739  PMID: 2155032

Abstract

The effects of cholesterol on the dynamics of cholestane spin probe (CSL) in various phosphatidylcholine-cholesterol mixed model membranes are examined. The lateral diffusion, D of CSL in DMPC/POPC/cholesterol ternary mixtures, is measured utilizing an improved dynamic imaging electron spin resonance method. It shows a factor of two decrease at 10 mol % and 25 degrees C, whereas it shows only a 40% decrease at 50 mol % and 50 degrees C. A comparison with results in POPC/cholesterol mixtures, which show a stronger effect of cholesterol on D, indicates that acyl chain unsaturation leads to stronger self association of cholesterol in PC model membranes. An S2CSL dependence of the activation energy for D, has been confirmed for the DMPC/POPC/cholesterol mixtures. Here SCSL is the order parameter for CSL. A similar correlation of R perpendicular, the perpendicular component of the rotational diffusion coefficient, with SCSL, which is true for all three mixtures (DMPC/cholesterol, POPC/cholesterol, and DMPC/POPC/cholesterol) we have studied, is also found. These are associated with the effects of enhanced local ordering on the free volume needed for translation and reorientation. Such correlations of dynamic properties D and R perpendicular with the thermodynamic quantity S, as well as the consistent interpretations of the effect of acyl chain unsaturation on the dynamics in terms of the activity coefficients, strongly emphasize the interrelation between the dynamic structure and the thermodynamics of the PC/cholesterol mixtures.

Full text

PDF
447

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alecio M. R., Golan D. E., Veatch W. R., Rando R. R. Use of a fluorescent cholesterol derivative to measure lateral mobility of cholesterol in membranes. Proc Natl Acad Sci U S A. 1982 Sep;79(17):5171–5174. doi: 10.1073/pnas.79.17.5171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. De Kruyff B., Van Dijck P. W., Demel R. A., Schuijff A., Brants F., Van Deenen L. L. Non-random distribution of cholesterol in phosphatidylcholine bilayers. Biochim Biophys Acta. 1974 Jul 12;356(1):1–7. doi: 10.1016/0005-2736(74)90288-0. [DOI] [PubMed] [Google Scholar]
  3. Jost P. C., Griffith O. H. The molecular reorganization of lipid bilayers by osmium tetroxide. A spin-label study of orientation and restricted y-axis anisotropic motion in model membrane systems. Arch Biochem Biophys. 1973 Nov;159(1):70–81. doi: 10.1016/0003-9861(73)90430-x. [DOI] [PubMed] [Google Scholar]
  4. Kar L., Ney-Igner E., Freed J. H. Electron spin resonance and electron-spin-echo study of oriented multilayers of L alpha-dipalmitoylphosphatidylcholine water systems. Biophys J. 1985 Oct;48(4):569–595. doi: 10.1016/S0006-3495(85)83814-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Korstanje L. J., Van Faassen E. E., Levine Y. K. Slow-motion ESR study of order and dynamics in oriented lipid multibilayers: effects of unsaturation and hydration. Biochim Biophys Acta. 1989 Apr 14;980(2):225–233. doi: 10.1016/0005-2736(89)90403-3. [DOI] [PubMed] [Google Scholar]
  6. Kusumi A., Subczynski W. K., Pasenkiewicz-Gierula M., Hyde J. S., Merkle H. Spin-label studies on phosphatidylcholine-cholesterol membranes: effects of alkyl chain length and unsaturation in the fluid phase. Biochim Biophys Acta. 1986 Jan 29;854(2):307–317. doi: 10.1016/0005-2736(86)90124-0. [DOI] [PubMed] [Google Scholar]
  7. Lindblom G., Johansson L. B., Arvidson G. Effect of cholesterol in membranes. Pulsed nuclear magnetic resonance measurements of lipid lateral diffusion. Biochemistry. 1981 Apr 14;20(8):2204–2207. doi: 10.1021/bi00511a020. [DOI] [PubMed] [Google Scholar]
  8. Merkle H., Subczynski W. K., Kusumi A. Dynamic fluorescence quenching studies on lipid mobilities in phosphatidylcholine-cholesterol membranes. Biochim Biophys Acta. 1987 Feb 26;897(2):238–248. doi: 10.1016/0005-2736(87)90420-2. [DOI] [PubMed] [Google Scholar]
  9. Presti F. T., Chan S. I. Cholesterol-phospholipid interaction in membranes. 1. Cholestane spin-label studies of phase behavior of cholesterol-phospholipid liposomes. Biochemistry. 1982 Aug 3;21(16):3821–3830. doi: 10.1021/bi00259a016. [DOI] [PubMed] [Google Scholar]
  10. Recktenwald D. J., McConnell H. M. Phase equilibria in binary mixtures of phosphatidylcholine and cholesterol. Biochemistry. 1981 Jul 21;20(15):4505–4510. doi: 10.1021/bi00518a042. [DOI] [PubMed] [Google Scholar]
  11. Rubenstein J. L., Smith B. A., McConnell H. M. Lateral diffusion in binary mixtures of cholesterol and phosphatidylcholines. Proc Natl Acad Sci U S A. 1979 Jan;76(1):15–18. doi: 10.1073/pnas.76.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shimshick E. J., McConnell H. M. Lateral phase separation in phospholipid membranes. Biochemistry. 1973 Jun 5;12(12):2351–2360. doi: 10.1021/bi00736a026. [DOI] [PubMed] [Google Scholar]
  13. Shin Y. K., Freed J. H. Dynamic imaging of lateral diffusion by electron spin resonance and study of rotational dynamics in model membranes. Effect of cholesterol. Biophys J. 1989 Mar;55(3):537–550. doi: 10.1016/S0006-3495(89)82847-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shin Y. K., Freed J. H. Thermodynamics of phosphatidylcholine-cholesterol mixed model membranes in the liquid crystalline state studied by the orientational order parameter. Biophys J. 1989 Dec;56(6):1093–1100. doi: 10.1016/S0006-3495(89)82757-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Van Dijck P. W., De Kruijff B., Van Deenen L. L., De Gier J., Demel R. A. The preference of cholesterol for phosphatidylcholine in mixed phosphatidylcholine-phosphatidylethanolamine bilayers. Biochim Biophys Acta. 1976 Dec 2;455(2):576–587. doi: 10.1016/0005-2736(76)90326-6. [DOI] [PubMed] [Google Scholar]
  16. Vaz W. L., Clegg R. M., Hallmann D. Translational diffusion of lipids in liquid crystalline phase phosphatidylcholine multibilayers. A comparison of experiment with theory. Biochemistry. 1985 Jan 29;24(3):781–786. doi: 10.1021/bi00324a037. [DOI] [PubMed] [Google Scholar]
  17. Verkleij A. J., Ververgaert P. H., de Kruyff B., Van Deenen L. M. The distribution of cholesterol in bilayers of phosphatidylcholines as visualized by freeze fracturing. Biochim Biophys Acta. 1974 Dec 24;373(3):495–501. doi: 10.1016/0005-2736(74)90029-7. [DOI] [PubMed] [Google Scholar]

Articles from Biophysical Journal are provided here courtesy of The Biophysical Society

RESOURCES