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Adherence of biotin-labeled recombinant SOWp58 to mammalian ECM proteins in a microtiter plate assay. Bound protein was detected spectrophometrically by secondary reaction with peroxidase-conjugated streptavidin plus substrate. BSA was used as a control. The bars represent mean values ± the standard deviation of three separate experiments. The difference in relative binding of rSOWp58 to laminin versus fibronectin was statistically significant (P = 0.0011).
