FIG. 2.

Effects of cytochalasin D and genistein on S. aureus internalization into alveolar epithelial cells. SV40-AT2 cells were cocultured with 8325-4 in the presence of inhibitors for 2 h (black bars). SV40-AT2 cells were then washed and reincubated for an additional 2 h or 4 h (diagonal stripes) in the presence of only S. aureus. The extent of internalization was compared at each time point with that of SV40-AT2 cells cultured with S. aureus alone. Data are presented as the ratio of S. aureus internalized in the presence of inhibitor to S. aureus internalized in the absence of inhibitor. The experiment was performed twice with similar results. Data from one experiment are shown. Both inhibitors significantly reduced the extent of 8325-4 internalization into SV40-AT2 cells in comparison to SV40-AT2 cells cocultured with just 8325-4. ∗∗, P < 0.01 versus SV40-AT2 cells cocultured with 8325-4.