TABLE 4.

Overall expression and cellular localization of the OspE-related, OspF-related, and Elp lipoproteins in temperature-shifted and DMC-cultivated B. burgdorferi

Antibody	Lipoprotein expression (%)a
	Temperature shifted			DMC cultivated
	Total	Surface	% Surface	Total	Surface	% Surface
OspA	77.7	73.0	92.0	3.3b	0c	0d
OspC	74.3	64.3	86.9	93.7b	21.0c	22.3d
OspE	91.3	80.0	87.8	75.7b	51.3c	67.8
p21	9.7	4.3	45.5	38.3b	15.3c	40.7
OspF/BbK2.11	100	93.3	93.3	76.7b	31.7c	41.2d
ElpA2	61.3	42.7	71.1	33.3b	8.3c	26.2d
ElpB1	93.3	11c	11.7	76.3b	47.7c	62.5d
ElpB2	10.7	5.3c	38.4	46b	19c	40.5

^aThe standard deviations were less than 9% for all samples. The percentage of expression for each lipoprotein was determined from three independent experiments.

^bThe total percentage of DMC-cultivated spirochetes expressing the lipoprotein was significantly different from the percentage of temperature-shifted organisms expressing the lipoprotein (P ≤0.05).

^cThe percentage of the population with surface-exposed lipoprotein was significantly less than the total percentage of organisms expressing the lipoprotein (P ≤0.05).

^dThere was a significant difference in the percentages of DMC-cultivated and temperature-shifted organisms expressing the lipoprotein on the surface.