TABLE 4.
IdeR-independent, iron-repressed genesa
| Rv no. | Gene | mRNA ratio (WT LI/WT HI) | Gene productb |
|---|---|---|---|
| 0279c | PE-PGRS | 2.2 ± 0.2 | PE-PGRS protein |
| 0464c | 2.2 ± 0.2 | CHP | |
| 0465c | 4.3 ± 0.7 | Probable transcriptional regulator (PbsX/Xre family) | |
| 0467 | icl | 3.5 ± 0.4 | Isocitrate lyase |
| 0676c | mmpL5 | 2.8 ± 0.2 | Conserved large membrane protein |
| 0677c | mmpS5 | 3.2 ± 0.3 | Conserved small membrane protein |
| 0692 | 2.7 ± 0.2 | CHP | |
| 0693 | pqqE | 2.5 ± 0.3 | Coenzyme PQQ synthesis protein E |
| 0694 | lldD1 | 2.3 ± 0.2 | l-Lactate dehydrogenase (cytochrome) |
| 1169c | 2.2 ± 0.2 | PE protein | |
| 1184c | 2.2 ± 0.1 | CHP | |
| 1195 | 2.2 ± 0.2 | PE protein | |
| 1393c | 2.4 ± 0.3 | FAD-containing monooxygenase | |
| 1461 | 4.7 ± 0.4 | CHP | |
| 1462 | 3.4 ± 0.6 | CHP | |
| 1463 | 3.3 ± 0.5 | Probable ABC transporter | |
| 1464 | 3.0 ± 0.3 | NifS-like protein | |
| 1465* | 2.6 ± 0.5 | NifU-like protein | |
| 1466 | 3.2 ± 0.6 | CHP | |
| 1520 | 2.1 ± 0.2 | Probable glycosyltransferase | |
| 2621c | 2.4 ± 0.3 | Probable transcriptional regulator | |
| 2794c | 2.7 ± 0.2 | CH; similar to proteins involved in vibriobactin and enterobactin synthesis | |
| 3229c | desA3 | 5.0 ± 2.7 | Acyl[ACP] desaturase |
| 3230c | 2.5 ± 0.5 | Probable oxygenase | |
| 3614c | 2.1 ± 0.1 | CHP | |
| 3854c | ethA | 4.4 ± 0.4 | Probable monooxygenase |
A DNA microarray was used to measure mRNA levels in cultures of M. tuberculosis H37Rv during exponential growth under iron-deficient (2 μM FeCl3) (LI) or -sufficient (50 μM FeCl3) (HI) conditions and in exponential cultures of strains ST22 and ST52 in iron-sufficient medium. Differences in gene-specific RNA levels of twofold or more are listed. Genes contiguous to included genes and with a ratio of > 1.6 after the standard deviation was subtracted are also listed. Iron-dependent, IdeR-independent genes were derepressed under low-iron conditions in the wild-type (WT LI/HI > 2) and were not affected in their repression under high-iron conditions in the ideR mutant. All these genes showed a WT HI/ST22 HI ratio of 1.0 (not shown). The genes are annotated as described by the Pasteur Institute on TUBERCULIST (http://genolist.pasteur.fr/Tuberculist), except for that indicated by an asterisk. That gene is annotated based on an individual BLAST search. Genes that are separated by <50 bp and are probably coregulated are grouped.
CHP, conserved hypothetical protein; CHMP, conserved hypothetical membrane protein.