TABLE 5.

IdeR-independent iron-induced genes^a

Rv no.	Gene	mRNA ratio (WT HI/LI)	Gene productb
0706	rplV	1.6 ± 0.1	50S ribosomal protein L22
1252c	IprE	1.6 ± 0.1	Lipoprotein
1305	atpE	1.6 ± 0.1	ATP synthase c chain
1908c	KatG	1.7 ± 0.2	Catalase-peroxidase
1943c		1.7 ± 0.1	CHP
2526		1.6 ± 0.1	CHP
2549c		1.6 ± 0.1	Unknown
2550c		2.0 ± 0.2	Unknown
2741	PE-PGRS	2.0 ± 0.1	PE-PGRS subfamily
2927c		1.6 ± 0.1	CHP
3075c		1.7 ± 0.2	CHP
3145	nuoA	1.7 ± 0.3	NADH dehydrogenase chain A
3146	nuoB	1.8 ± 0.1	NADH dehydrogenase chain B
3147	nuoC	1.6 ± 0.2	NADH dehydrogenase chain C
3148	nuoD	1.7 ± 0.2	NADH dehydrogenase chain D
3152	nuoH	1.8 ± 0.1	NADH dehydrogenase chain H
3153	nuoI	1.6 ± 0.1	NADH dehydrogenase chain I
3155	nuoK	1.6 ± 0.2	NADH dehydrogenase chain K
3156	nuoL	1.7 ± 0.2	NADH dehydrogenase chain L
3157	nuoM	1.6 ± 0.1	NADH dehydrogenase chain M
3158	nuoN	1.6 ± 0.1	NADH dehydrogenase chain N
3246	mtrA	2.0 ± 0.3	Two-component response     regulator
3394		1.7 ± 0.2	Unknown

^aA DNA microarray was used to measure mRNA levels in cultures of M. tuberculosis H37Rv during exponential growth under iron-deficient (2 μM FeCl₃) (LI) or -sufficient (50 μM FeCl₃) (HI) conditions and in exponential cultures of strains ST22 and ST52 in iron-sufficient medium. Genes induced by 1.6-fold or more are listed. Iron-dependent, IdeR-independent genes were induced under high-iron conditions in the wild type (WT HI/LI > 1.6), and their expression under high-iron conditions was not affected in the ideR mutant. All these genes showed a WT HI/ST22 HI ratio of 1.0 (not shown). The genes are annotated as described by the Pasteur Institute on TUBERCULIST (http://genolist.pasteur.fr/Tuberculist). Genes that are separated by <50 bp and are probably coregulated are grouped.

^bCHP, conserved hypothetical protein; CHMP, conserved hypothetical membrane protein.