FIG. 2.

Effect of T. cruzi GIPL on LPS-stimulated macrophages. (A) Macrophages were incubated with Y strain GIPL (50 μg/ml) for 3 h before or after LPS addition or simultaneously added to LPS (500 pg/ml). (B, C, and D) Macrophages obtained from PBMC were kept unstimulated (Unst.), incubated with LPS alone (500 pg/ml), or incubated with GIPL (50 μg/ml) plus LPS (500 pg/ml) for 48 h. After this period, supernatants were harvested and their contents were analyzed by ELISA for determination of the production of IL-10 (B), IL-12p40 (C), and IL-8 (D). The data shown are from a single experiment, representative of five separate experiments.