FIG. 4.

Effect of T. cruzi GIPL on LPS-stimulated DC. DC obtained from PBMC from healthy volunteers (see Material and Methods) were kept unstimulated (Unst.), incubated with LPS alone (500 pg/ml), or incubated with GIPL (50 μg/ml) plus LPS (500 pg/ml) for 48 h. After this period, supernatants were harvested and their contents were analyzed by ELISA for determination of the production of TNF-α (A), IL-10 (B), IL-12p40 (C), and IL-8 (D). The data shown are from a single experiment, representative of five separate experiments.