FIG. 6.

Ceramide moiety from T. cruzi GIPL (G strain) inhibited LPS-induced TNF-α secretion from macrophages. Macrophages obtained from PBMC were incubated with LPS alone or with GIPL (50 μg/ml) or isolated ceramide (12.5 μg/ml) plus LPS (500 pg/ml) for 48 h. After this period, supernatants were harvested and their contents were analyzed by ELISA determination of the production of TNF-α. The data shown are from a single experiment, representative of five separate experiments.