TABLE 1.

Primers used for PCR

Gene	Orientationa	Sequence (5′ to 3′)b
hTLR4	upper-1	AT GGA TCC ACC ATG ATG TCT GCC TCG CGC CTG
	upper-2	AC CGA AGC TTG TCC TGC GTG AGA CCA GAA AGC TG
	lower	TAA ATT CTC GAGTCA GAT AGA TGT TGC TTC CTG CCA
mTLR4	upper-1	GAA GGA TCCACC ATG ATG CCT CCC TGG CTC CTG
	upper-2	GGG GTA CCG TCC TGC CTG ACA CCA GGA AGC TTG
	lower	C AGA GTT TGC GGC CGCTCA GGT CCA AGT TGC CGT TTC TTG
hMD-2	upper-1	G GAA TTC ACC ATG TTA CCA TTT CTG TTT TTT TCC ACC
	upper-2	ACC GAA GCT TTG GAA GCT CAG AAG CAG TAT TGG GTC
	lower	GC TCT AGACTA ATT TGA ATT AGG TTG GTG TAG GAT
mMD-2	upper-1	G GAA TTC ACC ATG TTG CCA TTT ATT CTC TTT TCG ACG
	upper-2	AT TAA AGC TTG GAA TCT GAG AAG CAA CAG TGG
	lower	CCG CTC TAG ATT TTT TTT TTT TTT TTT Tc

^aThe upper-1 and upper-2 primers were used to amplify the entire coding region and the coding region minus the signal peptide sequence, respectively.

^bSequences in italics and boldface indicate restriction sites created and the respective coding regions, respectively.

^cSince the 3′ portion of the mMD-2 sequence had not been determined at the time of creation, an oligo(dT) primer was used.