FIG. 5.

T-cell-polarizing ability of alternatively activated macrophages in vivo during T. crassiceps infection. Adherent peritoneal macrophages were obtained from infected mice at the times indicated, as well as from age-matched uninfected mice. Cells (10⁶) were loaded in vitro with 50 μg of OVA for 2 h. CD4⁺ T cells (2 × 10⁶) from healthy mice previously immunized with OVA were cocultured, and 72 h later supernatants were analyzed for IFN-γ (a), IL-12 (b), IL-4 (c), and IL-6 (d). Data are representative of three independent experiments with at least four animals individually assayed. Error bars indicate ± standard deviations. Asterisk, P < 0.05 comparing with macrophages from healthy mice or early (2 weeks)-infected mice.