Figure 1.

Transgene tissue expression. (A) SYN-LEPR-B transgene expression analysis in several tissues of Syn-db3J/db3J mice. A bp fragment of approximately 1100 is generated from tissue cDNA when the SYN-LEPR-B transgene is expressed. (B) Expression of Hprt is used as a loading control. Fragments from Hprt generate a 195-bp fragment. Lanes correspond to tissues in A and B. Hth, hypothalamus; WAT, white adipose tissue; Ctx, cerebral cortex; BAT, brown adipose tissue; Pit, pituitary gland; Ov, ovary; Panc, pancreas; Te, testes; Liv, liver; Adr, adrenal gland; Mus, skeletal muscle; Blk, blank. gDNA is used as a control for genomic DNA contamination.