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. 2005 Nov 10;115(12):3484–3493. doi: 10.1172/JCI24059

Figure 2.

Figure 2

Transgene expression in the PVN. In situ hybridization of LEPR-B mRNA in hypothalamic brain sections, including the PVN. Counterstained sections are represented in A, C, E, and G, and in situ hybridization autoradiographic images are represented in B, D, F, and H. Genotypes are indicated. A and B represent +/+ mice, C and D represent Syn db/db mice, E and F represent Nse db/db mice, and G and H represent Nse+Syn db/db mice. The NSE-LEPR-B transgene (F) and wild-type LEPR-B (B) are weakly expressed in the PVN when compared with the SYN-LEPR-B transgene (D). The complementation of expression pattern is evident in (H), where both transgenes are coexpressed in Nse+Syn db/db mice. Magnification, ×20.