Fig. 2. Pt@BSA-RM combined with X-ray irradiation showed the best proliferation inhibition and apoptosis promotion effects in BC cells. (A and B) Immunofluorescence analysis of EdU incorporation in BC cells in the control, treated with Pt@BSA alone, and treated with Pt@BSA-RM alone or cisplatin groups as well as these treatments in combination with X-ray irradiation (n = 3) (scale bar = 100 μm) groups. (C) Cell proliferation from the above cells measured via CCK-8 analysis (n = 3). (D and E) Representative flow cytometry images for each group and the percentage of apoptotic cells (n = 3). Data are presented as mean ± SD. *p < 0.05; ***p < 0.001; ***p < 0.0001; ns, no significance.