Fig. 4. Pt@BSA-RM exacerbated X-ray-induced mitochondrial damage and inhibited glycolysis. (A) Mitochondrial platinum accumulation detected by GFAAS (n = 3). (B) Detection of the mitochondrial potential using the JC-1 assay (JC-1 aggregate, red; JC-1 monomer, green) (scale bar = 100 μm). (C) Intracellular ATP levels in BC cells detected using the ATP assay (n = 3). (D) Extracellular lactate levels in BC cells detected using the lactic acid assay (n = 3). (E) Western blot analysis showing the HK2, PKM2, and LDH1 protein expressions in different groups (n = 3). (F) Relative mRNA expression of HK2, PKM2, and LDH1 in different groups (n = 3). (G) ECAR of BC cells detected as an indicator of the deduced glycolysis flux and glycolytic capacity in the control group, after treatment with Pt@BSA alone and Pt@BSA-RM alone and these treatments in combination with X-ray irradiation (n = 3). (H) OCR detected as an indicator for oxidative phosphorylation (OXPHOS) in BC cells in the different groups (n = 3). Data are presented as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, no significance.