TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype | Source or reference |
|---|---|---|
| Y. enterocolitica | ||
| JB580v | Serogroup O:8, Nalr, ΔyenR (R−, M+) | 16 |
| GY460 | ΔflhDC | 35 |
| GY455 | crp::TnMod-RKm′ | This study |
| GY562 | crp::TnMod-RKm′ | This study |
| GY572 | cya::TnMod-RKm′ | This study |
| GY654 | cya::TnMod-RKm′ | This study |
| YEDS10 | yplA-lacZY (pFUSE), yplA+ | 26 |
| GY4455 | yscK::TnMod-lacZYA-RKm′ | This study |
| GY4641 | ysaE::TnMod-lacZYA-RKm′ | K. Venecia and G. M. Younga |
| GY4531 | crp::str | This study |
| GY4651 | yscK::TnMod-lacZYA-RKm′, crp::str | This study |
| GY4659 | ysaE::TnMod-lacZYA-RKm′, crp::str | This study |
| GY843 | flhB-lacZYA (pFUSE), flhB+ | This study |
| GY4681 | flhB-lacZYA (pFUSE), flhB+, crp::str | This study |
| E. coli | ||
| S17-1 λpir | recA thi pro hsd (R− M+) RP4::2-Tc::Mu::Km Tn7 λpir | 27 |
| Plasmids | ||
| pGY355 | cya cloned into the EcoRI site of pTM100, Tcr | This study |
| pGY345 | crp cloned into the EcoRI site of pTM100, Tcr | This study |
| pGY362 | cya PCR product cloned into pCR-Blunt II-TOPO, Kmr | This study |
| pGY426 | crp PCR product cloned into pCR-Blunt II-TOPO, Kmr | This study |
| pGY364 | crp cloned into the suicide plasmid pEP185.2 | This study |
| pGY365 | crp::str cloned into the suicide plasmid pEP185.2 | This study |
| pEP185.2 | mob+, oriR6K, Cmr | 16 |
| pTM100 | mob+, derivative of pACYC184, Cmr Tcr | 19 |
| pCR-Blunt II-TOPO | Kmr | Invitrogen |
| pTnMod-RKm′ | TnMod-RKm′ in a delivery vector | 11 |
| pTnMod-lacZYA-RKm′ | pTnMod-RKm′ containing a promoterless lacZYA | Andrew Darwin |
| p34S-Sm | Plasmid containing a streptomycin resistance cassette | 11 |
a
Unpublished data.