| Method |
Vapor diffusion, sitting drop |
Vapor diffusion, sitting drop |
| Temperature (K) |
290 |
290 |
| Protein concentration (mg ml−1) |
24.8 |
24.8 |
| Buffer composition of protein solution |
20 mM HEPES pH 7.0, 0.3 M NaCl, 5%(v/v) glycerol, 1 mM TCEP |
20 mM HEPES pH 7.0, 0.3 M NaCl, 5%(v/v) glycerol, 1 mM TCEP |
| Volume and ratio of drop |
0.4 µl protein + 0.4 µl reservoir (1:1) |
0.4 µl protein + 0.4 µl reservoir (1:1) |
| Volume of reservoir (µl) |
80 |
80 |
| Composition of reservoir solution |
JCSG+ screen condition H10: 0.1 M bis-Tris buffer pH 5.5, 0.2 M ammonium acetate, 25%(w/v) PEG 3350 |
Morpheus screen condition H2: 10%(w/v) PEG 8000, 20%(w/v) ethylene glycol, 0.02 M of each amino acid, 0.1 M MES/imidazole pH 6.5 supplemented with 5 mM NAD+
|
| Composition of cryoprotectant |
Reservoir solution supplemented with 5%(v/v) ethylene glycol |
Direct cryoprotection from reservoir solution |
