Table 3. Data-collection and processing statistics.
Values in parentheses are for the outer shell.
| Apo BoSDR (PDB entry 5er6) | BoSDR–NAD+ (PDB entry 5ha5) | |
|---|---|---|
| Diffraction source | 21-ID-F, APS | 21-ID-F, APS |
| Wavelength (Å) | 0.97856 | 0.97872 |
| Temperature (K) | 100 | 100 |
| Detector | Rayonix MX-225 | Rayonix MX-225 |
| Space group | P1211 | P1211 |
| Crystal-to-detector distance (mm) | 140 | 170 |
| Rotation range per image (°) | 1 | 1 |
| Total rotation range (°) | 240 | 180 |
| Exposure time per image (s) | 10 | 10 |
| a, b, c (Å) | 52.01, 94.81, 99.49 | 52.50, 96.82, 101.90 |
| α, β, γ (°) | 90, 99.54, 90 | 90, 100.75, 90 |
| Mosaicity (°) | 0.234 | 0.271 |
| Resolution range (Å) | 50.00–1.55 | 50.00–1.90 |
| Total No. of reflections | 576794 | 296987 |
| No. of unique reflections | 135447 | 78788 |
| Completeness (%) | 98.3 (97.0) | 99.9 (99.9) |
| Multiplicity | 4.26 (4.29) | 3.77 |
| 〈I/σ(I)〉 | 12.18 (2.88) | 15.52 (2.72) |
| CC1/2 (%) | 99.7 (80.3) | 99.8 (82.8) |
| Rp.i.m. (%) | 3.62 (24.8) | 4.29 (25.4) |
| Overall B factor from Wilson plot (Å2) | 13 | 19 |