TABLE 2.

Effect of MPL on B7-1 and B7-2 expression on murine splenic macrophages^a

MPL (ng/ml)	Mean fluorescence intensityb
	B7-1	B7-2
0	575 ± 49	259 ± 47
100	1,844 ± 196**	358 ± 24**
1,000	1,644 ± 144**	339 ± 24
10,000	779 ± 123	342 ± 21*

^aSingle spleen cell cultures were incubated in the presence or absence of MPL for 48 h. Cells were harvested and stained with allophycocyanin-conjugated anti-CD11b and FITC-labeled anti-CD86 or PE-labeled anti-CD80 antibody and analyzed by flow cytometry. Values are the mean ± SEM.

^b*, significantly different from control (no MPL) at P < 0.01; **, significantly different from control (no MPL) at P < 0.001.