FIG. 2.
Confirmation of gene arrangement within the msa-2 locus. B. bovis Mo7 DNA was treated with BamHI plus BglII (lane 1) or BamHI (lane 2) and analyzed by Southern blotting with digoxigenin-labeled oligonucleotide probes corresponding in lane 1 to the initial 18 5′ nt of msa-2a1, -2a2, and -2b (oligonucleotide msa-2-F1 in Table 1) or in lane 2 to the initial 18 5′ nt of msa-2c (oligonucleotide msa-2c-F in Table 1). Hybridization was detected by chemiluminescence. Size markers in kilobases are shown on the left.