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. 2002 Jul;70(7):3566–3575. doi: 10.1128/IAI.70.7.3566-3575.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 6. — Reactivity of postinfection and monospecific serum antibodies against msa-2 gene products. (A) Immunoblots showing the reactivity of bovine preimmune and anti-Mo7 postinfection immune serum (1:500) against recombinant MSA-2 antigens. (B) Anti-Mo7 immune serum was blocked with each purified recombinant MSA-2 antigen as indicated, and the reactivity was tested against the different rMSA-2 antigens as in panel A. (C) Immunoblot showing the reactivity of MSA-2a₁ monospecific, polyclonal serum antibodies and MAb 23/70.174 with native B. bovis merozoite antigens. Lane 1, control mouse serum; lane 2, polyclonal mouse serum against rMSA-2a₁; lane 3, MAb 23/70.174; lane 4, polyclonal mouse serum against rMSA-2b run as a size marker; lane 5, control MAb Tryp1E1. The position of the 45-kDa molecular size marker is indicated on the right.