FIG. 7.
Amino acid conservation between MSA-2 peptides from the B. bovis Mo7 and R1A strains. DNA from the Argentina R1A strain was amplified by PCR with primers msa-2-F1 and B42/44R for the msa-2a1, -2a2, and -2b genes and msa-2c-F and B42/44R for msa-2c. Amplification products were cloned, and several clones were sequenced to identify R1A orthologues of msa-2a1, -2a2, -2b, and -2c based on the degree of sequence identity. The figure shows alignments for each gene product. Areas of amino acid identity are enclosed in black boxes, conservative amino acid substitutions have a gray background, and variant amino acids have a white background. Deletions are indicated with dashed lines. The repeat region in MSA-2a1 and -2a2 (bold line above sequence), conserved YYKK sequence (bracket and ∗), and recombination sites between MSA-2a2 and -2b (∗∗) are indicated.