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CD40L titration in Leishmania-infected human DCs. DCs were infected with L. major (Lm) strain V1, L. tropica (Lt) strain KK27, or L. donovani (Ld) strain 9515 or stimulated with IFN-γ as a positive control. Infected or IFN-γ-treated cells were stimulated with different amounts of CD40L (0 to 4 μg/ml), and IL-12p70 secretion was assessed by ELISA. The values represent means ± standard errors of the means (n = 3 to 9 different donors).
