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. Author manuscript; available in PMC: 2026 Jan 19.
Published in final edited form as: Nat Protoc. 2024 Feb 2;19(5):1311–1347. doi: 10.1038/s41596-023-00948-y

Fig. 3 |. Comparison study of the glycolysis stress test in live cells by Seahorse Analyzer or FiLa.

Fig. 3 |

a,b, General overview of the procedure for the glycolysis stress test in live cells by Seahorse Analyzer (a) or FiLa (b). c, A glycolysis stress test was carried out by measuring the ECAR with Seahorse XF96 analyzer following sequential injections of glucose (Glc, 1 mM), oligomycin (Oligo, 1 μM) and 2-DG (50 mM) in HEK-293 cells (n = 9). d, Glycolytic parameters, including glycolysis, glycolytic capacity and glycolytic reserve, were calculated (n = 9). e–j, Fluorescence images (e,g and i) and quantification (f,h and j) of FiLa expressed in the cytosol (e and f) and nucleus (g and h) or FiLa-L in mitochondria (i and j) of HEK-293 cells. Cells were successively treated with 1 mM glucose, 1 μM oligomycin and 50 mM 2-DG at the indicated times. Scale bars, 10 μm. Data are presented as the mean ± s.e.m. (c and d).