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. 2002 Aug;70(8):4261–4272. doi: 10.1128/IAI.70.8.4261-4272.2002

FIG. 3.

FIG. 3.

PCR analyses of sequences upstream of the plasmid cpe gene of type A isolates. (A) Results of a dcm-specific PCR assay (expected ∼0.4-kbp product marked by arrow) with primers internal to F4969 dcm sequences. (B) Results of a PCR assay performed with primers designed to amplify a product from the DNA region between dcm sequences and sequences ∼400 bp downstream of them (based upon sequencing of the cpe-containing XbaI fragment of F4969). In this PCR assay, F4969 DNA yields an ∼0.9-kbp PCR product (small arrow), which is consistent with sequencing results for the F4969 plasmid cpe locus; note that one cpe-negative type A isolate, 297442, gave an ∼2.0-kbp product (large arrow). (C) PCR analysis of the association between dcm gene and IS1469 genes. An ∼2.1-kbp product (arrow) was detected in all type A isolates carrying a plasmid cpe gene. (D) PCR analysis of the association between dcm sequences and the cpe gene, which is consistent with sequencing results for the plasmid cpe locus of F4969. An ∼3.5-kbp product (arrow) was detected in all type A isolates carrying a plasmid cpe gene, which is consistent with sequencing results for the plasmid cpe locus of F4969. Numbers on the left indicate migration of molecular size markers.