TABLE 1.
ELISA reactivity of antisera raised against recombinant PorB protein preparationsa
| Formulation | Geometric mean ELISA titer (10−3) measured against:
|
|||
|---|---|---|---|---|
| Homologous rPorB protein | OMs from strain:
|
|||
| H44/76 | MC139 | MC114 | ||
| rPorB-Alum | 984 | 14 | - | - |
| Liposome preparations | ||||
| rPorB-liposomes | 12 | 1 | - | - |
| rPorB+MPLA-liposomes | 506 | 67 | 5 | - |
| rPorB-liposomes+MPLA- liposomes | 31 | 10 | - | - |
| Micelle preparations | ||||
| rPorB-Zwit micelles | 420 | 28 | 3 | - |
| rPorB+MPLA-Zwit micelles | 2,139 | 105 | 9 | - |
| rPorB-NDSB micelles | 145 | 4 | - | - |
| rPorB+MPLA-NDSB micelles | 4,915 | 143 | 17 | - |
a
Mice were immunized with recombinant PorB protein in the preparations as described in Materials and Methods. Pre- and postimmune sera were tested by ELISA against homologous protein and against outer membranes from the homologous PorB3 strain (H44/76), a heterologous PorB3 strain (MC139), and a heterologous PorB2 strain (MC114). Data are the geometric mean titers from groups of five mice. -, No reactivity was detected. Preimmune sera and antisera raised against control preparations lacking rPorB showed no reactivity (<1) against the antigens in ELISA.