FIG. 3.

Immunofluorescence analysis of fixed and permeabilized versus nonpermeabilized AT-treated tachyzoites. Tachyzoites were treated for 240 min with 10 nM AT, fixed, and processed for immunofluorescence with (+TX-100) or without (−TX-100) detergent (Triton X-100) permeabilization. Parasites were dual labeled with MAbs against the surface protein SAG1 and the dense granule protein GRA6 (A) or SAG1 and IMC1 (B). Arrows indicate toxin-induced membrane blebs, and arrowheads point to sites at which plasma membrane had ruptured. Scale bar, 5 μm.