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. 2025 Aug 29;4(1):54–64. doi: 10.1021/envhealth.5c00171

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© 2025 The Authors. Co-published by Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, and American Chemical Society

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Histomorphological and neuronal analysis of hippocampal slices from offspring brains via H&E staining and Nissl staining. (A) H&E staining and Nissl staining were used to stain hippocampal CA1, CA3, and DG regions, respectively. Scale bars: 100 μm in the overview hippocampal neuronal sections across distinct regions and 50 μm in the magnified views of dashed-box regions. CA1: cornu ammonis 1, CA3: cornu ammonis 3, and DG: dentate gyrus. (B) Neuronal counting of hippocampal slices from the brains of offspring.