FIG. 4.

Recall response to M. tuberculosis antigen by splenectomized or mock-splenectomized mice. At various times after infection, cell suspensions were prepared from the PLN (A), lungs (B), or spleens (C) of B6 mice which had been splenectomized or mock splenectomized prior to inoculation. The amounts of IFN-γ produced after 48 h of stimulation with a 1:5,000 dilution of M. tuberculosis sonicate are shown. The error bars indicate the standard deviations based on three to six mice per group. The spleens and LN were assayed individually. The lung mononuclear cells were pooled; thus, there are no error bars in panel B. The data are representative of the data obtained in two experiments.