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. 2002 Aug;70(8):4510–4522. doi: 10.1128/IAI.70.8.4510-4522.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 8. — (A and B) Alignment of the two best-conserved blocks characteristic of GAA-1. The protein evolved considerable divergence at the amino acid level, and only seven positions are invariant in the two aligned blocks (taken together) with the species A. thaliana (At), H. sapiens (Hs), S. cerevisiae (Sc), and P. falciparum (Pf). Accession numbers are CAA55944 (Sc), NP_197414 (At), and AAH03171 (Hs). (C and D). The histidine (C) and cysteine (D) in the boxed motifs of GPI8 are essential for the transamidation reaction (see text) in other species and are conserved in P. falciparum. Accession numbers are AAB81597 (Hs), P49018 (Sc), T40853 (S. pombe [Sp]), and AJ401202 (Pf). CS, consensus sequence. Symbols and figure preparation are as described in the legend to Fig. 3.