FIG. 9.

Global alignment of the DPM1 protein sequences from A. thaliana (At), H. sapiens (Hs), and P. falciparum (Pf). The alignment shows a high degree of overall conservation. The only gaps are a short deletion in the Arabidopsis gene near the presumptive start codon and a one-amino-acid deletion at position 24 in the Plasmodium protein. The second aspartate in the boxed IDDGS motif and the first aspartate in the boxed MDAD motif are seen in a range of β-glycosyltransferases that transfer a single sugar unit and that have been suggested to participate in catalysis (53). Also boxed is a conserved GTRY motif, where the R is invariant in a broad family that includes sequences of unknown function annotated as DPM1-like proteins in databases. Accession numbers are AAH07073 (Hs) and NP_173481 (At). CS, consensus sequence. Symbols and figure preparation are as described in the legend to Fig. 3.