. 2002 Aug;70(8):4510–4522. doi: 10.1128/IAI.70.8.4510-4522.2002

TABLE 2.

Sequences of intron-exon boundaries of the P. falciparum genes

Gene	In- tron	Length (bp)	Sequence^a
Gene	In- tron	Length (bp)	Splice donor	Splice acceptor
GAA-1	1	172	`AAGTTATAGG gttagtaaag aaaaaaaaaa`	`atttgatata tctctttttt aatgtttcat acatttttag CGTTTTCTTA`
	2	318	`ACTTTCTGAG gtacaaaaaa aaatatgaat`	`tttatttatt tatacatttc ttttgtgttt atccctttag AGAGAACATT`
	3	219	`ATTAAGTTCG gtaagtaacc ctaaaataaa`	`tatattttta tttttttttt ttatattcct ttttttttag TTATATAATT`
PIG-O	1	160	`ATATACTAAA gtaatacaat ccacacatta`	`tacatttata tatatttata aatttttgtt ccttttgtag AGCCTTCATT`
	2	225	`TGTAATACAT ctataaaaga aaaattttat`	`ttttttataa cgggacataa atttatttta aataacttac CACTAGTTTC`
PIG-A	1	209	`ATAAAAAAGG gttatacata taaaaaaaaa`	`taatatttgt ttcaaatatt ttatttttat ttttttttag GTTTCAAGGT`
	2	123	`TGGTCACCAG gtgcaagaac aaataaataa`	`tttatttatt tatttattat tattattatt atttttttag GCTACGTCAG`
	3	206	`CCACGAAAGG gtaaacatga gtttattaac`	`gttcatattt tttttttttt tttttttttt ttttatctag CTAACCAAAA`
	4	131	`GGAAAAGACG gtaaaatcac ataatacata`	`gtttttatgt ttattcttgt ttcattgttt tataatatag GAAAAGGTGT`
	5	86	`ACAGTTTTTA gtaatataaa aaataatata`	`tttatttata tatatatata tatattttcc attattgtag GCATAATTTA`
	6	147	`TCATGCCAAG gcaagattaa aaagaagaaa`	`atatataagc ctattcatgt ttcctttttt ttttttttag ACAAAATATT`
PIG-M	1	150	`TCTATATCAT gtaagtattc aaatatatgt`	`ttttaaaaga atacattatt attttttttt tttatatcag ATATGGATAT`
	2	124	`GAATTCAAAG gtaataataa taataaaaaa`	`tatatatata tatatatata tgttgttttt ttctttaaag ATTATTCCCT`
	3	78	`GTTTTTACAA gtatataata attaatatat`	`aatatatata tatatatata tatgtgtgtg ttttttttag ACTATTTCTT`
	4	179	`CACATCTCAG gtaaataaag gagaaattaa`	`ctacaaattt catataattt atatttaatt tattttttag TATTTCATTT`
	5	103	`CTTAAGCAAG gtaataaaaa aaaaagaaaa`	`atatatatat atatatatta tttttttttt tttaatgcag AGAAATATGT`
	6	169	`TGTGGCAAAA gtgggctcaa aaagatttct`	`tactacatat gtattatatt atattttatt ttatttttag TTGCATTGGC`
	7	85	`CTTTTTACAA gtaatgaatt tctttatttt`	`ttttataata tatattcttt tattctcaca tattttatag TTATTTTACT`
Consensus			`*******AG gtaaaaaaa aaaaaaaa`	`ttttt ttttttt tttttttttt ttttttttag *********T`

The exon is in uppercase, and the intron is in lowercase. There are two noncanonical splice sites (boldface). A GC donor in the PIG-A gene and a very unusual CT-AC second intron in the PIG-O gene are shown. The last line shows the consensus across these 18 introns. The consensus was defined when residues occurred in at least 51% of the aligned sequences; otherwise, positions are indicated by asterisks.