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Effect of an antioxidant, NAC, on R. rickettsii-induced HO-1 mRNA expression. EC were left untreated (Con) or preincubated with NAC (500 μM) for 60 min. (NAC), followed by infection with R. rickettsii in its absence (RR) or presence (RR+NAC) for 4 h. Total RNA isolated from EC for each experimental condition was resolved by electrophoresis and the blot was hybridized with HO-1 and GAPDH probes, respectively. The results shown are representative of two independent experiments.
