FIG. 2.

Genetic organization of the Y. pestis ybtD region showing restriction sites used. (A) Dashed line indicates the region deleted in the ΔybtD mutant. The PCR product used in complementation studies is also indicated. The BamHI and XbaI sites in parentheses are artificial restriction sites introduced by PCR. (B) Putative −10 and −35 regions, potential ribosomal binding sites (RBS), and a region with similarity to a Fur binding site (underlined nucleotides), as well as the potential protein start sites, are indicated (underlined and in boldface type). Arrows show the two promoter regions tested in expression studies.