Reactivity of 12 M. catarrhalis strains with Hag-specific MAb 5D2. Whole-cell lysates of the following M. catarrhalis wild-type isolates were probed in a Western blot analysis with Hag-reactive MAb 5D2: O35E (lane 1), O12E (lane 2), O46E (lane 3), ATCC 25238 (lane 4), ATCC 25240 (lane 5), P44 (lane 6), TTA24 (lane 7), TTA37 (lane 8), E22 (lane 9), V1171 (lane 10), 4223 (lane 11), ETSU-13 (lane 12), and ETSU-25 (lane 13). Molecular size position markers (in kilodaltons) are shown on the left.