FIG. 8.

Use of cryoimmunoelectron microscopy to detect UspA1, UspA2, and Hag on the M. catarrhalis cell surface. Biofilm-derived cells of uspA2 hag double mutant O35E.2HG, expressing only the UspA1 protein (A and B), uspA1 hag double mutant O35E.1HG, expressing only the UspA2 protein (C and D), and uspA1 uspA2 double mutant O35E.ZC, expressing only the Hag protein (E and F) were probed with UspA1- and UspA2-reactive MAb 17C7 (A, C, and E) and with Hag-specific MAb 5D2 (B, D, and F) prior to incubation with gold particle-conjugated goat anti-mouse IgG. Bars (all panels), 100 nm.