FIG. 3.

Physical and genetic map and partial nucleotide sequence of the recB gene from BF2, MC58, and BF10. (A) Physical and genetic map of the genomic recB region from strains MC58 and BF2. The black box within the recB gene of BF2 indicates the region that contains many missense mutations. The map of the recB region from MC58 was deduced on the basis of the available sequencing data (36). The homologous region was sequenced from strain BF2 (accession number AF495855). The position of the recB-specific probe used in the Southern blot experiments (Fig. 2) is also indicated below the map. Abbreviations: DUS, DNA uptake sequence; SRE, neisserial small repetitive element; orf, open reading frame. (B) Alignment of partial nucleotide sequences of the recB coding region from strains BF2, MC58, and BF10. The sequences of the recB coding region containing missense mutations in BF2 and BF10 (from nucleotides 601 to 951) are presented.