FIG. 1.
VEGF (A), IL-1β (B), and IL-8 (C) induction in THP-1 macrophages infected with viable or heat-killed B. henselae (Bh). Cells were seeded into 24-well plates at a concentration of 106 cells/well. THP-1 cells were differentiated by treatment with PMA. After a 2-day resting period, PMA-differentiated, adherent cells were infected at an MOI of 1,500. Heat-killed bacteria were boiled for 30 min prior to coculture with THP-1. Supernatants were analyzed by ELISA. The data depicted correspond to a representative experiment from three trials. Values are means and standard deviations; asterisks indicate statistical significance (P < 0.05).