FIG. 2.
VEGF (A), IL-1β (B), and IL-8 (C) induction in B. henselae (Bh)-infected THP-1 macrophages pretreated with cytochalasin D (CyD). THP-1 cells were seeded into 24-well plates at a concentration of 106 cells/well and differentiated into macrophages by treatment with PMA. After a 2-day resting period, PMA-differentiated, adherent cells were infected at a MOI of 100. Selected groups were treated with 1 μg of CyD per ml prior to infection. Supernatants were collected at the indicated times and analyzed by ELISA for protein expression. The data depicted correspond to a representative experiment from three trials. Values are means and standard deviations; asterisks indicate statistical significance (P < 0.05). DMSO, dimethyl sulfoxide.